STUDY OF ACUTE AND SUB-ACUTE TOXICITY OF A PREPARATION BASED ON SULFADIMIDINE AND TRIMETHOPRIM

In Ukraine, the market of veterinary drugs is saturated with a large number of preparations of various purposes, a significant share of which is occupied by sulfonamides. The combination of sulfanilamide (sulfadimidine) and trimethoprim provides an opportunity to manifest the drug to exhibit a bactericidal effect. At the same time, the appearance of strains of microorganisms resistant to the action of the drug is insignificant. At the same time, the use of medicinal products with several active substances in veterinary practice requires careful study of the toxicity of complex preparations. The parameters of the acute toxicity of the preparation "Riketron N" were studied on white rats, 2-3 months old, weighing 180-200 g. The preparation was administered intramuscularly, once. To determine the toxicity of the preparation "Riketron N" for white rats, doses of 1000, 2500 and 5000 mg/kg of the animal's body weight were taken. Six laboratory animals were used for each dose. Subacute toxicity was studied on 18 white rats weighing 180-200 g. For this purpose, according to the principle of analogues, a control and two experimental groups of animals, 6 rats in each, were formed. The preparation was administered intramuscularly. Animals of the control group were injected with water for injections. The animals of the 1st experimental group were administered the preparation "Riketron N" in a therapeutic dose of 0,03 ml per animal; the animals of the 2nd experimental group received the preparation in a 5-fold therapeutic dose of 0,15 ml per animal. In a subacute experiment, the preparation "Riketron N" was administered to rats for 10 days. Under the conditions of intramuscular administration of the preparation "Riketron N", the DL50 for white rats is 1583 mg/kg of body weight. The use of the drug "Riketron N" in therapeutic and 5-fold therapeutic doses for 10 days leads to liver and kidney damage in all experimental animals.