SIMULTANEOUS DETERMINATION OF COCCIDIOSTATICS IN FEEDS AND PREMIXES USING HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY

Coccidiostats – ionophore polyester antibiotics (monensin sodium, lasalocid sodium, salinomycin sodium, narazin, maduramycin ammonium and others) have a wide spectrum of action. Coccidiostats are used for the treatment and prevention of eimeriosis in target animals. They are added to the feed in the appropriate dosage. They are used to increase the efficiency of growth and development of farm animals and poultry also. Production of such feeds and premixes requires strict control of possible processes of cross-contamination with other coccidiostats, safety and quality. Salinomycin sodium, monensin sodium, narazin and maduramycin ammonium in feed and premix samples were determined by high-performance thin-layer chromatography (HPTLC). The separation is carried out due to the dissolution of the investigated substances in the mobile phase and is based on adsorption, distribution, ion exchange and the combination of these processes in the stationary phase. This technique proved to be a sufficiently specific and economically advantageous express method for the determination of coccidiostats. In addition, analyzed substances are determined simultaneously by the method of high-performance thin-layer chromatography, up to 17 samples can be analyzed on one plate under the same conditions. This is important for optimizing working time in the laboratory and efficient use of consumables. Two-dimensional separation mixture standard samples of coccidiostats was carried out to check the stability of the analytes in the stationary phase on the plate. The limit of detection of the studied analytes by the HPTLC method was 3.0 mg/kg. Were analyzed 199 feed samples and 266 premix samples of monensin sodium, salinomycin sodium, narasin and maduramycin ammonium content from 2015 to 2024 years. The samples were sent to National Reference Laboratory of Veterinary Drug Residues Control for the State Registration of Veterinary Drug, Feed Additives and for Contractual Subjects. Therefore, the coccidiostats detection and determination of their content in feed and premixes for the control of processes cross-contamination with other coccidiostats, for safety and for quality remains relevant in the future and will continue.