PATHOGNOMONIC TRANSFORMATIONS IN ANTS IN THE BIOASSEMBLY OF GENERALIZED TUBERCULOSIS INFECTION

Tuberculosis is an emergent zoonotic mycobacterial disease that affects humans and farm animals, including dairy cows that produce milk that may contain pathogens of lethal infectious diseases. The insidiousness of tuberculosis is that infected cows in the initial period of the disease appear conditionally healthy externally and, being a permanent source of infectious prokaryote for a long time, months and years, can be in a state of latent course of the disease and produce organoleptically unchanged, but infected milk. Tuberculosis mycobacteria are highly specialized bactopathogens that have adapted to intracellular endoparasitism and therefore do not have a rich exofermentative apparatus for extracellular catabolism. As a result, they do not cause spoilage of milk as saprophytic forms of banal microflora, but when non-disinfected native milk is used as food, they are able to take root in the human body and cause emergent infectious diseases with unpredictable negative consequences for the health of consumers of dietary livestock products. Timely indication of the causative agent of tuberculosis in milk and the body of animals is an urgent task of preventive epizootic monitoring of the biosafety of agricultural animal exploitation and prevention of the occurrence and spread of zoonotic infectious diseases. Bacteriological methods are traditionally used to isolate the causative agent of tuberculosis, but the environmental plasticity of the causative agent leads to false-negative results, so it is necessary to improve the means of indicating pathogenic mycobacteria, both in livestock products and in the body of farm animals. The biological method of indicating the causative agent of tuberculosis on the model of ants is one of the most sensitive and it is theoretically equal to the effectiveness of PCR-on line. The epizootic culture of M. bovis isolated from milk by a biological method on ants was isolated on Stonebrink's elective-selective medium and the main basic properties were studied. Pathogenic mycobacteria possessed typical biological properties, induced the genoform of tuberculosis in ants with pathognomonic transformations in the lungs, liver and spleen, sufficient for pathomorphological diagnosis and characteristic pathohistological changes in the internal organs of laboratory animals.