ASSESSMENT OF THE SUITABILITY OF THE METHOD FOR DETERMINING THE CONTENT OF LACTIC ACID IN FEED ADDITIVES USING THE CAPILLARY ELECTROPHORESIS METHOD

The article presents a review on the use of technological feed additives in feed for farm animals and poultry, justification of the need according to current regulatory documents, based on certain categories and functional groups of feed additives. Also the expediency of using lactic acid, its properties and methods of use in various areas, such as air disinfection, equipment and inventory in livestock and poultry farms, and in combination with such a substance as iodotriethylene glycol for the prevention of respiratory diseases in calves and respiratory infections in chickens, were noted. The positive influence of lactic acid on the fermentation process and the production of high-quality wet fodder for farm animals, the mechanisms of action and the antimicrobial, bactericidal and fungicidal properties of this organic acid were noted. The need to control lactic acid in technological feed additives, which are added to feed in order to improve their efficiency when feeding farm animals and poultry, is substantiated. In the "Materials and methods" section, the implementation and assessment of the suitability of the method of determining the content of lactic acid in feed additives by the capillary electrophoresis method with the help of the Kapel-105M capillary electrophoresis system «Elforan» computer support is presented. Also, the analysis scheme, applied test conditions, concentrations of calibration solutions, used standard sample for analysis are given. This section lists the validation characteristics that were established to assess the suitability of this technique for determining the content of lactic acid in feed additives. The obtained results of the validation characteristics studies show that the method of determining the content of lactic acid in feed additives using the capillary electrophoresis method meets the requirements of international standards of the ISO 5725 series regarding the results of validation when assessing the suitability of the test method. The calibration characteristics of this test method satisfy the conditions for this method, namely: the correlation coefficient is greater than 0.99, and the value of deviations at each point does not exceed 10%. The technique is reproducible, meets the requirements for convergence, accuracy and linearity with the use of Fisher coefficients. The reproducibility limit r for the probability Р = 0.95 of lactic acid for the study of the feed additive is 11.0%, the relative value of the permissible discrepancy between the two results of determinations obtained in two different laboratories - the reproducibility limit R for the probability Р = 0.95 is 19.0%, the average accuracy of the test method is 99.7%, the expanded relative uncertainty of the method with the coverage factor k=2**, Uvid, is 15.0%. Therefore, the obtained results indicate that the method of determining the content of lactic acid in feed additives by the capillary electrophoresis method is suitable for controlling the content of lactic acid in feed additives.