<i>ClBRN1</i> from <i>Chrysanthemum lavandulifolium</i> enhances the stress resistance of transgenic <i>Arabidopsis</i>
Bioinformatics analysis showed that the <i>ClBRN1</i> gene was a member of the NAC TFs family, with a CDS (coding sequence) length of 1,080 bp and encoding 359 amino acids. The subcellular localization results found that the gene was located in the nucleus and cell membrane. Furthermore, the transgenic results in <i>Arabidopsis thaliana</i> showed that the gene significantly reduces plant height while improving salt and low temperature tolerance. Observation of paraffin sections of <i>Arabidopsis</i> stems also revealed that the secondary cell wall of overexpressing <i>Arabidopsis</i> stems was significantly thicker than that of wild-type. The above results indicate that the <i>ClBRN1</i> gene may play an important role in regulating plant resistance to abiotic stress. This study will provide new insights for molecular breeding of resistant chrysanthemums in the future.
Authors
- Hai Wang (ORCID: https://orcid.org/0000-0002-4192-5363)
- Yueyue Liu
- Yanxi Li
- Wenting He
- Chendi Mei
- Xuebin Song
Institutions
- Qingdao Agricultural University (CN)
Publication Details
- Journal
- PeerJ
- Published
- 2024-12-12
- DOI
- https://doi.org/10.7717/peerj.18620
- Citations
- 1
- Primary Topic
- Plant Gene Expression Analysis
- Type
- article
- Field-Weighted Citation Impact
- 0.23
Funders
- National Natural Science Foundation of China