Neuronal synaptic architecture revealed by cryo-correlative light and electron microscopy
Cryo-correlative light and electron microscopy (cryo-CLEM) is a powerful technique that combines fluorescence imaging for specific localization with electron microscopy for detailed structural analysis, enabling high-resolution exploration of synaptic structures in neurons. In this study, we employed a cryo-CLEM approach using three independent alignment markers to precisely correlate electron microscopy (EM) images with light microscopy (LM) images of neuronal synapses under cryogenic conditions. This methodology revealed a distinctive pattern of electron densities in the synaptic clefts. Additionally, we were able to capture high-resolution images of presynaptic vesicles in various states, underscoring the potential of cryo-CLEM in advancing synaptic research.
Authors
- Wenhao Sun
- Yuchen Yao (ORCID: https://orcid.org/0000-0002-4154-1829)
- Yanhong Xue (ORCID: https://orcid.org/0000-0003-3214-4175)
- Huiqin Luan (ORCID: https://orcid.org/0000-0002-4598-6470)
- Shuli Zhang (ORCID: https://orcid.org/0000-0001-8006-1134)
- Buyun Tian (ORCID: https://orcid.org/0000-0002-0027-5499)
- Xiaojun Xu (ORCID: https://orcid.org/0000-0002-0127-2812)
- Liqiao Hu (ORCID: https://orcid.org/0000-0003-3904-4070)
- Weixing Li (ORCID: https://orcid.org/0000-0001-9215-0708)
- Yuanyuan Li (ORCID: https://orcid.org/0000-0002-1497-6376)
- Xia Li (ORCID: https://orcid.org/0000-0001-5478-8970)
- Yan Zhang
- Pei Wang (ORCID: https://orcid.org/0000-0001-5612-705X)
- Wei Ji (ORCID: https://orcid.org/0000-0002-2317-9053)
- Wei Feng
Institutions
- Chinese Academy of Sciences (CN)
- Institute of Biophysics (CN)
- National Research Center for Rehabilitation Technical Aids (CN)
- University of Chinese Academy of Sciences (CN)
Publication Details
- Journal
- Biophysics Reports
- Published
- 2024-12-12
- DOI
- https://doi.org/10.52601/bpr.2024.240035
- Citations
- 2
- Primary Topic
- Advanced Electron Microscopy Techniques and Applications
- Type
- article
- Field-Weighted Citation Impact
- 1.04