Practical advice for extracellular vesicle isolation in plant–microbe interactions: Concerns, considerations, and conclusions
In recent years, extracellular vesicles (EVs) have emerged as novel key players in plant-microbe interactions. While it is immensely useful to draw on the established "minimal information for studies of extracellular vesicles" (MISEV) guidelines and precedents in mammalian systems, working with plants and their associated microbes poses specific challenges. To navigate researchers through these obstacles, we offer detailed step-by-step suggestions for those embarking on EV research in the context of plant-microbe interactions. The advice is based on recent publications and our collective experience from the diverse plant and microbe systems studied in a dedicated research consortium. We provide considerations for experimental design, optimization, quality control, and recommendations on how to increase yield, purity, and reproducibility of EV isolation. With this perspective article, we aim not only to assist researchers in our field but also to promote discussions on plant and microbe EVs in the broader EV community.
Authors
- Sabrine Nasfi (ORCID: https://orcid.org/0000-0001-5916-0824)
- Hannah Thieron (ORCID: https://orcid.org/0000-0002-0670-7328)
- Alessa Ruf (ORCID: https://orcid.org/0000-0003-0919-9717)
- Ralph Panstruga (ORCID: https://orcid.org/0000-0002-3756-8957)
- Silke Robatzek (ORCID: https://orcid.org/0000-0002-9788-322X)
- Lorenz Oberkofler (ORCID: https://orcid.org/0000-0002-9506-2751)
- Seomun Kwon (ORCID: https://orcid.org/0000-0002-0514-1465)
- Laura Krassini
- Arne Weiberg (ORCID: https://orcid.org/0000-0003-4300-4864)
- Michael Feldbrügge (ORCID: https://orcid.org/0000-0003-0046-983X)
- Julia Kehr (ORCID: https://orcid.org/0000-0003-3617-9981)
- Karl‐Heinz Kogel (ORCID: https://orcid.org/0000-0003-1226-003X)
- Susanne Fricke
Institutions
- Universität Hamburg (DE)
- Justus-Liebig-Universität Gießen (DE)
- Cluster of Excellence on Plant Sciences (DE)
- Heinrich Heine University Düsseldorf (DE)
- Ludwig-Maximilians-Universität München (DE)
- RWTH Aachen University (DE)
Publication Details
- Journal
- Journal of Extracellular Vesicles
- Published
- 2024-12-01
- DOI
- https://doi.org/10.1002/jev2.70022
- Citations
- 9
- Primary Topic
- Extracellular vesicles in disease
- Type
- article
- Field-Weighted Citation Impact
- 2.10
Funders
- Deutsche Forschungsgemeinschaft
- European Research Council