Context-Dependent Estrogenic Actions of (+)-Pisatin Produced in Elicited Green or Snow Pea (<i>Pisum sativum</i>)
Legumes are a predominant source of isoflavones, termed phytoestrogens, that mimic 17β-estradiol (E2). Phytoalexins are inducible isoflavones produced in plants subjected to environmental stressors (e.g., UV, heat, or fungi). This study investigated estrogenic activity of snow and green peas elicited with <i>Aspergillus sojae</i>. Elicited extracts increased estrogenic activity and proliferation of breast cancer cells (MCF-7 or T47D) in a dose-dependent manner but exhibited antiestrogenic activity when combined with synthetic E2. HPLC analysis of elicited pea extracts identified (+)-pisatin as the primary phytoalexin, which was produced significantly (<i>p</i> < 0.0001) more in snow pea compared to green pea. RNA sequencing results suggested potential functional effects on endothelial cells and tissue vascularization. Indeed, (+)-pisatin enhanced metrics of network assembly and maturation in a microphysiological model of bulk tissue vasculogenesis. Thus, context-dependent functional effects of (+)-pisatin and pharmacologically similar phytoestrogens on the entire tissue microenvironment should be considered in preclinical investigation as potential therapeutic agents.
Authors
- Mark J. Mondrinos (ORCID: https://orcid.org/0000-0002-2704-9440)
- Van T. Hoang
- Jack D. North (ORCID: https://orcid.org/0009-0007-0481-2304)
- Brian G. Rowan
- John A. McLachlan (ORCID: https://orcid.org/0000-0002-9039-1361)
- Sophie R. Dietrich
- Jack Elliott
- Stephen M. Boué (ORCID: https://orcid.org/0000-0002-2790-4427)
- Matthew E. Burow (ORCID: https://orcid.org/0000-0002-0642-6630)
- Megan C. Benz
- Thomas Wiese (ORCID: https://orcid.org/0000-0002-1664-5917)
- Bridgette M. Collins‐Burow (ORCID: https://orcid.org/0000-0003-0039-6415)
- Jorge A. Belgodere (ORCID: https://orcid.org/0000-0003-3399-4796)
- Khoa Nguyen (ORCID: https://orcid.org/0000-0002-8059-6136)
- Muralidharan Anbalagan (ORCID: https://orcid.org/0000-0001-5797-7558)
- Syreeta L. Tilghman (ORCID: https://orcid.org/0000-0003-3215-1252)
- G. Wills Kpeli
- Elizabeth C. Martin (ORCID: https://orcid.org/0000-0002-8988-8151)
- Peng Ma (ORCID: https://orcid.org/0000-0003-3947-9146)
- Steven Elliott (ORCID: https://orcid.org/0000-0003-1167-867X)
- Thomas Cheng
- Binghao Zou (ORCID: https://orcid.org/0009-0008-4779-8183)
- Isaac J. Ponder
Institutions
- Tulane University (US)
- Louisiana State University (US)
- Agricultural Research Service (US)
- Louisiana State University Agricultural Center (US)
- Xavier University (US)
- Tulane Medical Center (US)
- Southern Regional Research Center (US)
- Pharmaceutical Biotechnology (Czechia) (CZ)
- Florida Agricultural and Mechanical University (US)
Publication Details
- Journal
- Journal of Agricultural and Food Chemistry
- Published
- 2024-12-12
- DOI
- https://doi.org/10.1021/acs.jafc.4c06409
- Citations
- 1
- Primary Topic
- Phytoestrogen effects and research
- Type
- article
- Field-Weighted Citation Impact
- 0.42
Funders
- U.S. Department of Agriculture
- Tulane University
- Louisiana State University
- National Institutes of Health
- Agricultural Research Service
- National Cancer Institute