Comparison of in Vitro Proliferation and Antioxidant Activity Between Ginseng-Derived Exosome-Like Nanoparticles and Ginseng Original Liquid

To compare the proliferation and antioxidant activity of Ginseng-derived exosome-like nanoparticles (GELNs) and Ginseng extracts (GEXs) in vitro. GEXs were prepared from natural plant ginseng, and GELNs were extracted by ultracentrifugation combined with sucrose density gradient centrifugation. The CCK-8 method was used to compare the effects of GELNs and GEXs on the proliferation of skin cells. The scratch test was used to compare the effects of GELNs and GEXs on the migration ability of skin cells, and then to verify the ability of GELNs and GEXs to promote proliferation, the antioxidant activity of GELNs and GEXs was compared by OH + DPPH + ABTS + FRAP method. The results showed that although GELNs and GEXs could promote the proliferation of human keratinocytes cells (HaCaT) at a certain concentration, GELNs not only showed better ability to promote the proliferation of skin cells, but also showed good biosafety. In addition, under the same protein concentration, GELNs showed stronger ability to scavenge OH, DPPH, ABTS free radicals and reduce iron ions than GEXs, that is, GELNs had relatively stronger antioxidant activity. The GELNs obtained by purification technology potentially improved the bioavailability and biosafety of ginseng, in order to provide a theoretical basis for its in-depth study as a natural antioxidant.