A colourimetric sensing based on MXene@Hemin nanoenzyme for the rapid detection of low-density lipoprotein

The increase of low-density lipoprotein (LDL) concentration will increase the cholesterol content in blood, which will pose a serious threat to vascular health and lead to a series of cardiovascular diseases (CVD) with high mortality. In this study, a two-dimensional titanium carbide@hemin nanoenzyme (MXene@Hemin) with good peroxidase activity was prepared, and its enzymatic activity was verified. Then, the sandwich colorimetric aptamer sensor of LDLApt/LDL/MXene@Hemin-LDL_Apt was constructed and applied to the detection of actual human serum. Under optimal experimental conditions, the colorimetric aptamer sensor showed a good linear relationship with LDL concentration from 0.5 to 80.0 μg/mL with a minimum detection limit(LOD) of 0.277 μg/mL. In the detection of LDL concentration in actual serum samples, the relative standard deviation (RSD) and relative error (SD) of the sensor are 0.641%~6.717% and 0.428%~3.205%, respectively. It has good sensitivity and specificity, and provides a simple and sensitive means for the prevention and evaluation of cardiovascular diseases.