Novel replication-competent reporter-expressing Rift Valley fever viruses for molecular studies
Rift Valley fever virus (RVFV) is a mosquito-borne virus and zoonotic agent threat that can be deadly to domestic or wild ungulates, and humans. In this work, we used reverse genetics approaches to explore the genome plasticity of RVFV by generating a set of recombinant (r)RVFV that express fluorescent or luminescent proteins to track viral infection. All the generated reporter-expressing rRVFVs were able to propagate in mammalian or insect cells and a mouse model of infection. Our studies may contribute to advances in research on RVFV and other bunyaviruses and pave the way for the development of novel vaccines and the identification of new antivirals for the prophylactic and therapeutic treatment, respectively, of RVFV infections.
Authors
- Belén Borrego (ORCID: https://orcid.org/0000-0002-0801-9166)
- Sandra Moreno (ORCID: https://orcid.org/0000-0002-3548-037X)
- Alejandro Brun (ORCID: https://orcid.org/0000-0001-7865-538X)
- Gema Lorenzo (ORCID: https://orcid.org/0000-0003-1869-9051)
- Aitor Nogales (ORCID: https://orcid.org/0000-0002-2424-7900)
- Luis Martínez‐Sobrido (ORCID: https://orcid.org/0000-0001-7084-0804)
- Celia Alonso (ORCID: https://orcid.org/0009-0009-9661-3226)
Institutions
- Texas Biomedical Research Institute (US)
- Consejo Superior de Investigaciones Científicas (ES)
- Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (ES)
Publication Details
- Journal
- Journal of Virology
- Published
- 2024-12-12
- DOI
- https://doi.org/10.1128/jvi.01782-24
- Citations
- 3
- Primary Topic
- Viral Infections and Vectors
- Type
- article
- Field-Weighted Citation Impact
- 1.17
Funders
- European Commission
- Ministerio de Economía y Competitividad
- HORIZON EUROPE Framework Programme
- European Regional Development Fund
- Agencia Estatal de Investigación