Direct measurement of DNA bending by quantum magnetic imaging of a nanomechanical torque balance
DNA flexibility is a key determinant of biological function, but the inability to directly measure the bending energy at short, biophysically relevant length scales leaves this quantity poorly understood. The authors measure DNA bending directly, by tethering a ferromagnetic nanoparticle probe to an individual DNA molecule, applying a magnetic field, and reading out a diamond magnetic field sensor. Wide-field imaging of quantum defects near the surface of the diamond enables measurement of the DNA bending torque.
Authors
- Kohei M. Itoh (ORCID: https://orcid.org/0000-0002-8153-4893)
- Hideyuki Watanabe (ORCID: https://orcid.org/0000-0003-3994-5610)
- Joseph Turnbull
- Isaac M. Shelby (ORCID: https://orcid.org/0000-0003-1567-8765)
- Ruhee Nirodi (ORCID: https://orcid.org/0000-0001-7266-7945)
- Zeeshawn Kazi (ORCID: https://orcid.org/0000-0002-9563-4334)
- Kai‐Mei C. Fu (ORCID: https://orcid.org/0000-0003-4775-8524)
- Paul A. Wiggins (ORCID: https://orcid.org/0000-0002-8316-4339)
Institutions
- Pacific Northwest National Laboratory (US)
- University of Washington (US)
- Keio University (JP)
- National Institute of Advanced Industrial Science and Technology (JP)
Publication Details
- Journal
- Physical Review Applied
- Published
- 2024-12-12
- DOI
- https://doi.org/10.1103/physrevapplied.22.064044
- Citations
- 1
- Primary Topic
- Force Microscopy Techniques and Applications
- Type
- article
- Field-Weighted Citation Impact
- 0.34
Funders
- National Science Foundation