Author Response: Levels of the HtrA1 Protein in Serum and Vitreous Humor Are Independent of Genetic Risk for Age-Related Macular Degeneration at the 10q26 Locus

recombinant HtrA1 in a linear manner in the SCTM ELISA.Despite this, there was no detectable signal when quantifying the MBS standards in the MBS diluents in the SCTM ELISA (see Supplementary Fig. S5).In conclusion, our article provides a robust validation of the SCTM ELISA and convincingly shows that neither the MBS1 assay nor the MBS2 assay is likely to detect native HtrA1.We invite Pan and Iwata 1 to reassess their original findings, 3 based on the MBS2 assay, using a validated and robust method to specifically and selectively quantify HtrA1 protein.Without this key validation step, a requisite for the scientific method, it is not possible to compare any HtrA1 ELISA with their results.