Optimized single-cell gates for yeast display screening
Yeast display is a widely used technology in antibody discovery and protein engineering. The cell size of yeast enables fluorescence-activated cell sorting (FACS) to precisely screen gene libraries, including for multi-parameter selection of protein phenotypes. However, yeast cells show a broader size distribution than mammalian cells that complicates single-cell gate determination for FACS. In this report, we analyze several yeast display gating options in detail and present an optimized strategy to select single yeast cells via flow cytometry. These data reveal optimized single-cell gating strategies to support robust and high-efficiency yeast display studies.
Authors
- Xiaoli Pan (ORCID: https://orcid.org/0000-0002-5033-7272)
- Jacy R. Wolfe (ORCID: https://orcid.org/0000-0001-5206-4135)
- Bailey B. Banach (ORCID: https://orcid.org/0000-0003-0208-5856)
- Bharat Madan (ORCID: https://orcid.org/0000-0002-7376-9991)
- Brandon J. DeKosky (ORCID: https://orcid.org/0000-0001-6406-0836)
- Aric Huang (ORCID: https://orcid.org/0000-0001-8203-4818)
- Azady Pirhanov (ORCID: https://orcid.org/0000-0002-1443-8228)
- Matheus Oliveira de Souza (ORCID: https://orcid.org/0000-0003-0357-1995)
- Francisco M Figueiras
Institutions
- University of Kansas (US)
- Ragon Institute of MGH, MIT and Harvard (US)
- Massachusetts General Hospital (US)
- Massachusetts Institute of Technology (US)
Publication Details
- Journal
- Protein Engineering Design and Selection
- Published
- 2024-12-12
- DOI
- https://doi.org/10.1093/protein/gzae018
- Citations
- 2
- Primary Topic
- Monoclonal and Polyclonal Antibodies Research
- Type
- article
- Field-Weighted Citation Impact
- 0.81
Funders
- Bill and Melinda Gates Foundation
- Massachusetts General Hospital
- Harvard T.H. Chan School of Public Health
- National Institutes of Health