Generation of a genetically encoded voltage indicator MARINA reporter human iPS cell line using Cas9 (VULSCi002-A-2)
Fluorescent protein-based Genetically Encoded Voltage Indicators (GEVI) offer a remarkable system for high-throughput screening of membrane potential phenotypes. The GEVI MARINA is a derivative from ArcLight, which conversely to ArcLight increases its fluorescence intensity alongside depolarization. Here we created knock-in reporter human iPS cell lines carrying the MARINA reporter using SpCas9 programmable nuclease and characterize a heterozygous clone.
Authors
- Daniel Naumovas (ORCID: https://orcid.org/0000-0002-5968-4182)
- Aurelijus Burokas (ORCID: https://orcid.org/0000-0002-0364-3496)
- Jonathan Arias (ORCID: https://orcid.org/0000-0002-3997-2355)
- Mindaugas Stoškus (ORCID: https://orcid.org/0000-0001-8947-0380)
- Artūras Jakubauskas (ORCID: https://orcid.org/0000-0002-6305-0617)
- Ivan Nalvarte (ORCID: https://orcid.org/0000-0001-6828-2583)
- Carlos Polanco (ORCID: https://orcid.org/0000-0003-1175-306X)
- Bárbara Rojas (ORCID: https://orcid.org/0000-0002-0697-0185)
- Daiva Baltriukienė (ORCID: https://orcid.org/0000-0002-7851-9270)
- Beatričė Valatkaitė-Rakštienė (ORCID: https://orcid.org/0009-0008-7915-4702)
- Laimonas Griškevičius (ORCID: https://orcid.org/0000-0002-3731-1537)
- Urtė Neniškytė (ORCID: https://orcid.org/0000-0001-7645-703X)
- Akshay Kumar Vijaya (ORCID: https://orcid.org/0009-0000-5106-7983)
- Patricia Muñoz (ORCID: https://orcid.org/0000-0001-6596-9451)
- José Inzunza (ORCID: https://orcid.org/0000-0003-0876-6767)
- Rimvydas Norvilas (ORCID: https://orcid.org/0000-0002-8502-0750)
- Aistė Petruškevičiūtė
- Ugnė Šimuliūnaitė
- Simonas Kuras
Institutions
- Vilnius University (LT)
Publication Details
- Journal
- Stem Cell Research
- Published
- 2024-12-13
- DOI
- https://doi.org/10.1016/j.scr.2024.103628
- Citations
- 1
- Primary Topic
- CRISPR and Genetic Engineering
- Type
- article
- Field-Weighted Citation Impact
- 0.23