Revealing extensive inbreeding and less efficient purging of deleterious mutations in wild Amur tigers in China
Inbreeding increases genome homozygosity within populations, which can exacerbate inbreeding depression by exposing homozygous deleterious alleles that are responsible for declines in fitness traits. In small populations, genetic purging that occurs under the pressure of natural selection acts as an opposing force, contributing to a reduction of deleterious alleles. Both inbreeding and genetic purging are paramount in the field of conservation genomics. The Amur tiger (Panthera tigris altaica) lives in small populations in the forests of Northeast Asia and is among the most endangered animals on the planet. Using genome-wide assessment and comparison, we reveal substantially higher and more extensive inbreeding in wild Amur tigers (F<sub>ROH</sub> = 0.50) than in captive individuals (F<sub>ROH</sub> = 0.24). However, a relatively reduced number of loss-of-function mutations in wild Amur tigers is observed compared to captive individuals, indicating genetic purging of inbreeding load with relatively large-effect alleles. The higher ratio of homozygous mutation load and number of fixed damaging alleles in the wild population indicates a less-efficient genetic purging, with purifying selection also contributing to this process. These findings provide valuable insights for the future conservation of Amur tigers.
Authors
- Shanlin Liu (ORCID: https://orcid.org/0000-0001-8118-8313)
- Weiyao Kong
- Yuan Fu
- Yinping Tian (ORCID: https://orcid.org/0000-0002-6998-0612)
- Yue Ma (ORCID: https://orcid.org/0000-0002-6555-6451)
- Minhui Shi (ORCID: https://orcid.org/0009-0002-9582-6018)
- Yanchun Xu (ORCID: https://orcid.org/0000-0003-0397-4292)
- Tianming Lan (ORCID: https://orcid.org/0000-0002-6934-0439)
- Love Dalén (ORCID: https://orcid.org/0000-0001-8270-7613)
- Qiye Li (ORCID: https://orcid.org/0009-0003-6681-519X)
- Le Zhang (ORCID: https://orcid.org/0000-0002-4378-6053)
- Nicolás Dussex (ORCID: https://orcid.org/0000-0002-9179-8593)
- Jiale Fan (ORCID: https://orcid.org/0000-0002-0453-7476)
- Guangshun Jiang (ORCID: https://orcid.org/0000-0001-6321-9489)
- Sunil Kumar Sahu (ORCID: https://orcid.org/0000-0002-4742-9870)
- Dan Liu (ORCID: https://orcid.org/0000-0002-2198-3910)
- Huan Liu (ORCID: https://orcid.org/0000-0003-3909-0931)
- Chen Lin (ORCID: https://orcid.org/0000-0001-9821-2578)
- Yue Zhao (ORCID: https://orcid.org/0000-0001-7712-6101)
- Haimeng Li (ORCID: https://orcid.org/0000-0001-6276-7125)
- Liangyu Cui
- Boyang Liu
Institutions
- BGI Group (China) (CN)
- Chinese Academy of Sciences (CN)
- Botswana Geoscience Institute (BW)
- Education Department of Heilongjiang Province (CN)
- Centre for Palaeogenetics (SE)
- Jilin Provincial Academy of Forestry Science (CN)
- Institute of Zoology (CN)
- Northeast Forestry University (CN)
- Heilongjiang University (CN)
Publication Details
- Journal
- Journal of genetics and genomics/Journal of Genetics and Genomics
- Published
- 2024-12-12
- DOI
- https://doi.org/10.1016/j.jgg.2024.12.004
- Citations
- 8
- Primary Topic
- Genetic Mapping and Diversity in Plants and Animals
- Type
- article
- Field-Weighted Citation Impact
- 4.40