Influence of sludge biochar at different carbonization temperatures on anammox process

Adding biochar can expedite the establishment of the anaerobic ammonia oxidation (anammox) process and improve the nitrogen removal efficiency of the anammox reactor. However, the optimization research of biochar derived from dewatered sludge on anammox is relatively limited. In this study, four sequencing batch reactors (SBRs) were compared for the enrichment of anammox bacteria using synthetic wastewater with sludge biochar carbonized at temperatures of 300°C, 550°C, and 800°C, and without biochar (CK). The start-up and the nitrogen removal performance of anammox process were evaluated, as well as the effect of organic carbon on nitrogen removal. The results showed that the addition of sludge biochar at different pyrolysis temperatures all can accelerate the start-up of the anammox process, improve the nitrogen removal efficiency, and reduce the total nitrogen (TN) in the effluent. Although the reactor with biochar carbonized at 800°C showed the fastest increase in the nitrogen loading, the best TNRE occurred in the reactor with biochar carbonized at 300°C, which was 8.0% higher than those of the control (CK, <i>p</i> < 0.05). The predominant genus of anammox in SBRs differed between the sludge biochar reactor and the control reactor (without biochar), which were <i>Candidatus Brocadia</i> and <i>Candidatus Jettenia</i>, respectively. Additionally, the total abundances of anammox bacteria and denitrifiers increased with the addition of sludge biochar.