Colicin Immunity Proteins of Pathogenic Bacteria Detected by Antibiotic‐Induced SOS Response, Plasmid Sequencing, MALDI‐TOF‐TOF Mass Spectrometry, and Top‐Down Proteomic Analysis
Our top-down proteomic method demonstrates that it is possible to screen putative pathogenic bacteria (whose genomes have been sequenced in full, in part or not at all) for the presence of phage- and plasmid-encoded toxin and colicin genes under SOS control. Small plasmid sequencing confirmed the presence of colicin/immunity genes (and their regulatory control) suggested from induction and top-down proteomic analysis.
Authors
- Clifton K. Fagerquist (ORCID: https://orcid.org/0000-0002-2598-3686)
- Jihyun Park (ORCID: https://orcid.org/0000-0002-1926-3607)
- Yanlin Shi
Institutions
- Oak Ridge Associated Universities (US)
- Agricultural Research Service (US)
- Western Regional Research Center (US)
- Oak Ridge Institute for Science and Education
Publication Details
- Journal
- Rapid Communications in Mass Spectrometry
- Published
- 2024-12-12
- DOI
- https://doi.org/10.1002/rcm.9964
- Citations
- 2
- Primary Topic
- Bacteriophages and microbial interactions
- Type
- article
- Field-Weighted Citation Impact
- 0.92
Funders
- U.S. Department of Energy
- U.S. Department of Agriculture
- Oak Ridge Associated Universities
- Oak Ridge Institute for Science and Education
- Agricultural Research Service