A Mn(salen)‐Based Artificial Metalloenzyme for Nitrene and Oxene Transfer Catalysis
The development of artificial metalloenzymes (ArMs) offers a potent approach to incorporate non-natural chemical reactions into biocatalysis. Here we report the assembly of Mn(salen)-based ArMs by embedding biotinylated Mn(salen) complexes into streptavidin (Sav) variants. Using commercially available nitrene and oxo transfer reagents, these biohybrid catalysts catalyzed the aziridination of alkenes and oxidation of benzylic C-H bonds with up to 19 and 146 turnover numbers.
Authors
- Ravi Kumar Verma (ORCID: https://orcid.org/0000-0002-4956-2763)
- Yi Ling Goh
- Hao Fan (ORCID: https://orcid.org/0000-0003-0199-9752)
- Yeuk Ting Law
- Barindra Sana
- Mun Fei Eddy Wong
- Yee Hwee Lim (ORCID: https://orcid.org/0000-0002-7789-3893)
- Zhennan Liu (ORCID: https://orcid.org/0000-0003-3341-0877)
Institutions
- Agency for Science, Technology and Research (SG)
- National University of Singapore (SG)
- Singapore Institute of Technology (SG)
- Singapore Institute for Clinical Sciences (SG)
- Institute for Sustainability (GB)
- Bioinformatics Institute (SG)
Publication Details
- Journal
- ChemBioChem
- Published
- 2024-12-12
- DOI
- https://doi.org/10.1002/cbic.202400774
- Primary Topic
- Synthesis and Catalytic Reactions
- Type
- article
- Field-Weighted Citation Impact
- 0.00