Potential of endophytic bacteria from Kailan plant roots in suppressing the growth of Sclerotium rolfsii (Sacc.)

The use of biological agents, such as endophytic bacteria, provides an environmentally friendly and safe method for controlling diseases caused by fungi. Sclerotium rolfsii (Sacc.), a fungus, causes damping-off disease in various horticultural plants, including chili plants. This study aimed to use endophytic bacteria from the roots of Kailan plants to suppress the growth of S. rolfsii. The research involved 1) isolating endophytic bacteria, 2) testing for biosafety, 3) propagating S. rolfsii, and 4) testing the antagonistic effects of the endophytic bacteria. The study found 36 isolates of endophytic bacteria. Bacterial colonies isolated from Kailan roots showed diversity in color and shape, with growth rates observed 48 hours post-isolation. Three isolates tested positive for hemolysis, and six isolates were hypersensitive. The antagonism test indicated that isolates grown on Tryptic soy agar (TSA) media demonstrated greater inhibitory effects on S. rolfsii than those on Potato dextrose agar (PDA) media, with a clearer inhibition zone appearing each day. In total, six isolates of endophytic bacteria from Kailan roots inhibited the growth of S. rolfsii.