Research on a Device for Fluorescence Imaging Detection of Nucleic

With the rapid development of nucleic acid molecule detection technology, nucleic acid detection devices have become a global research hotspot in recent years. This paper uses multidisciplinary fusion technology to propose a nucleic acid fluorescence detection design scheme. The device is mainly composed of a motion module and an optical system. Among them, the motion module uses an S-type variable acceleration control method to precisely control the movement of the motor. The stable and accurate operation lays the foundation for the collection of fluorescent signals; the optical system uses LED as the fluorescent excitation light source. The LED collimated uniform light path is designed. Use image stitching to stitch the collected images into a complete image for analysis. Finally, measure the operating error of the motor, the average error does not exceed 14um; simulate the fluorescence signal acquisition system and collect the image of the fluorescent film, the image fluorescence is uniform, which verifies the rationality of the fluorescence acquisition system; collects simulated samples and blank control images, Analyze the stitched images, and the results clearly distinguish the simulated samples and the control group.

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Publication Details

Published
2024-08-23
DOI
https://doi.org/10.1109/isset62871.2024.10779902
Primary Topic
Advanced Biosensing Techniques and Applications
Type
article
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article

Research on a Device for Fluorescence Imaging Detection of Nucleic

Yuanhua Yu, Luyang Duanmu
Advanced Biosensing Techniques and Applications
article

Research on a Device for Fluorescence Imaging Detection of Nucleic

Yuanhua Yu, Luyang Duanmu
article en

Abstract

With the rapid development of nucleic acid molecule detection technology, nucleic acid detection devices have become a global research hotspot in recent years. This paper uses multidisciplinary fusion technology to propose a nucleic acid fluorescence detection design scheme. The device is mainly composed of a motion module and an optical system. Among them, the motion module uses an S-type variable acceleration control method to precisely control the movement of the motor. The stable and accurate operation lays the foundation for the collection of fluorescent signals; the optical system uses LED as the fluorescent excitation light source. The LED collimated uniform light path is designed. Use image stitching to stitch the collected images into a complete image for analysis. Finally, measure the operating error of the motor, the average error does not exceed 14um; simulate the fluorescence signal acquisition system and collect the image of the fluorescent film, the image fluorescence is uniform, which verifies the rationality of the fluorescence acquisition system; collects simulated samples and blank control images, Analyze the stitched images, and the results clearly distinguish the simulated samples and the control group.

Changchun University of Science and Technology (CN)
Openalex Percentile: Top 80%
Advanced Biosensing Techniques and Applications
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