Research on a Device for Fluorescence Imaging Detection of Nucleic
With the rapid development of nucleic acid molecule detection technology, nucleic acid detection devices have become a global research hotspot in recent years. This paper uses multidisciplinary fusion technology to propose a nucleic acid fluorescence detection design scheme. The device is mainly composed of a motion module and an optical system. Among them, the motion module uses an S-type variable acceleration control method to precisely control the movement of the motor. The stable and accurate operation lays the foundation for the collection of fluorescent signals; the optical system uses LED as the fluorescent excitation light source. The LED collimated uniform light path is designed. Use image stitching to stitch the collected images into a complete image for analysis. Finally, measure the operating error of the motor, the average error does not exceed 14um; simulate the fluorescence signal acquisition system and collect the image of the fluorescent film, the image fluorescence is uniform, which verifies the rationality of the fluorescence acquisition system; collects simulated samples and blank control images, Analyze the stitched images, and the results clearly distinguish the simulated samples and the control group.
Authors
- Yuanhua Yu (ORCID: https://orcid.org/0000-0001-6447-5855)
- Luyang Duanmu
Institutions
- Changchun University of Science and Technology (CN)
Publication Details
- Published
- 2024-08-23
- DOI
- https://doi.org/10.1109/isset62871.2024.10779902
- Primary Topic
- Advanced Biosensing Techniques and Applications
- Type
- article
- Field-Weighted Citation Impact
- 0.00