Screening of bioactive components in Ferula assafo dried oleo-gum resin and assessment of its protective function against cadmium-induced oxidative damage, genotoxicity, and cytotoxicity in rats

Cadmium (Cd) is among the most ecologically harmful heavy metals. The purpose of this work was to identify the biologically active components in dried oleo-resin-gum of <i>Ferula assafo</i> extract (FAE) and assess their preventive efficacy against oxidative damage caused by Cd in rats. The biologically active components were identified using HPLC and GC-MS. Six groups of female Sprague-Dawley rats were randomly assigned and received oral treatment for two weeks. They consisted of the control group, the groups that got FAE at low or high doses (150 and 250 mg/kg b.w.), the group that received CdCl₂ (2 mg/kg b.w.), and the groups that received CdCl₂ + FAE at the low or high dose. Tissues and blood samples were collected for different assays and pathological examinations. The HPLC detected 11 polyphenol compounds, whereas the GC-MS identified 24 bioactive compounds. The <i>in vivo</i> study revealed that CdCl₂ alone disrupted all biochemical indices, oxidative indicators, cytokines, antioxidant enzymes, pro and anti-apoptotic mRNA gene expression, increased DNA fragmentation percentage, and caused pathological alterations in hepatic and renal sections. FAE plus CdCl₂ therapy considerably improved all indicators and the histological architecture of the kidney and liver, with the higher dose being more effective in improving all of the measured parameters. Therefore, FAE is a promising option for food and pharmaceutical applications to protect against oxidative damage caused by Cd exposure.