Electron Paramagnetic Resonance (EPR) Spectroscopic Study of a Phospholipid Bilayer Membrane: An Undergraduate Laboratory Experiment

This laboratory experiment aims to introduce undergraduate students to the use of electron paramagnetic resonance (EPR) spectroscopy as a valuable tool for studying lipid bilayer membranes in laboratory experiments. This spectroscopic method utilizes nitroxide spin-labeled stearic acid (5-DOXYL stearic acid) incorporated into a desired lipid bilayer membrane. Here, we highlight EPR’s pivotal role in investigating the dynamic properties of various membrane lipid bilayers and provide hands-on laboratory experience to upper-level chemistry/physics undergraduate students to develop biophysical and biochemical research skills. Undergraduate students will be immersed in wet lab knowledge and learn skills on operating a benchtop EPR instrument for EPR data acquisition, analyzing data, and writing a professional lab report.