Damage of the Bone Marrow Stromal Precursors in Patients with Acute Leukemia at the Onset of the Disease and During Treatment
In patients with acute leukemia (AL), malignant cells and therapy modify the properties of multipotent mesenchymal stromal cells (MSCs) and their descendants, reducing their ability to maintain normal hematopoiesis. The aim of this work was to elucidate the alterations in MSCs at the onset and after therapy in patients with AL. The study included MSCs obtained from the bone marrow of 78 AL patients (42 AML and 36 ALL) and healthy donors. MSC growth characteristics, gene expression pattern, proteome and secretome were studied using appropriate methods. The concentration of MSCs in the bone marrow, proliferative potential, the expression of several genes, proteomes and secretomes were altered in AL-MSCs. Stromal progenitors had been affected differently in ALL and AML patients. In remission, MSC functions remain impaired despite the absence of tumor cells and the maintenance of benign hematopoietic cells. AL causes crucial and, to a large extent, irreversible changes in bone marrow MSCs.
Authors
- Н. И. Дризе (ORCID: https://orcid.org/0000-0002-7150-0403)
- Е. Н. Паровичникова (ORCID: https://orcid.org/0000-0001-6177-3566)
- Maria A. Lagarkova (ORCID: https://orcid.org/0000-0001-9594-1134)
- Larisa A. Kuzmina (ORCID: https://orcid.org/0000-0001-6201-6276)
- Georgij Arapidi (ORCID: https://orcid.org/0000-0003-2323-1859)
- Luisa Karaseva
- Igor P. Smirnov (ORCID: https://orcid.org/0000-0003-0402-3392)
- Irina N. Shipounova (ORCID: https://orcid.org/0000-0003-1189-0283)
- Olga V. Pobeguts (ORCID: https://orcid.org/0000-0001-5265-7627)
- Olga Aleshina (ORCID: https://orcid.org/0000-0001-9924-0204)
- Н. А. Петинати (ORCID: https://orcid.org/0000-0001-6591-3183)
- Olga S. Pokrovskaya (ORCID: https://orcid.org/0000-0002-8657-4990)
- Anastasia Vasilieva
- Aleksandra Sadovskaya (ORCID: https://orcid.org/0000-0002-0221-9351)
Institutions
- Lomonosov Moscow State University (RU)
- National Medical Research Center for Hematology (RU)
- Institute of Bioorganic Chemistry (RU)
- Federal Medical-Biological Agency (RU)
Publication Details
- Journal
- International Journal of Molecular Sciences
- Published
- 2024-12-11
- DOI
- https://doi.org/10.3390/ijms252413285
- Citations
- 3
- Primary Topic
- Hematopoietic Stem Cell Transplantation
- Type
- article
- Field-Weighted Citation Impact
- 1.36
Funders
- Russian Science Foundation