DIAGNOSTICS OF CARRIERS OF HH2 FERTILITY HAPLOTYPES IN HOLSTIN COWS

The authors of the article used the Tetra-Primer ARMS-PCR reaction method to identify heterozygous carriers of the HH2 fertility haplotype in Holstein cows of foreign selection. A total of 150 DNA samples were tested, of which 8 individuals turned out to be heterozygous carriers of single-nucleotide deletion; the frequency of the harmful mutation was 5.4%. It has been established that the detection of DNA fragments on an electropherogram with sizes of 281 bp, 184 bp. and 145 bp, indicates that the animal is a heterozygous carrier of the HH2 fertility haplotype. It should be noted that to control the spread of harmful mutations, it is necessary to conduct selective genetic monitoring of the cattle population.