An enzyme-free immunosorbent assay of staphylococcal enterotoxin B using a three-way DNA junction amplifier with an automatic reset function
A highly sensitive immunoadsorption assay without traditional horseradish peroxidase for signal amplification was developed, utilizing a three-way DNA junction amplifier instead. The formation of a three-way DNA junction and release of trigger DNA with recycling was accomplished by toehold-mediated strand displacement. The fluorescent dye <i>N</i>-methyl mesoporphyrin IX, with highly structure-specific binding affinity towards G-quadruplexes, was employed for label-free and simple signal output. High sensitivity was achieved for concentrations as low as 7.21 pg mL<sup>-1</sup> by targeting Staphylococcal enterotoxin B for detection, and its applicability was confirmed by a recovery study of precooked dishes.
Authors
- Yahui Guo (ORCID: https://orcid.org/0000-0002-6512-355X)
- Yuliang Cheng (ORCID: https://orcid.org/0000-0002-9616-7133)
- Yunfei Xie (ORCID: https://orcid.org/0000-0002-2758-9236)
- W. D. Li (ORCID: https://orcid.org/0000-0002-9049-0685)
- Jinzhong Xi
- Zhilong Yu (ORCID: https://orcid.org/0000-0001-5828-2635)
- Weirong Yao (ORCID: https://orcid.org/0000-0003-4730-3976)
- Jiali Xing (ORCID: https://orcid.org/0000-0002-5731-0240)
- Shaofeng Yuan
- Hang Yu (ORCID: https://orcid.org/0000-0001-7927-0473)
Institutions
- Jiangnan University (CN)
- Ningbo Product Quality Supervision and Inspection Institute (CN)
- State Key Laboratory of Food Science and Technology (CN)
- Jiangsu Police Officer College (CN)
Publication Details
- Journal
- The Analyst
- Published
- 2024-12-11
- DOI
- https://doi.org/10.1039/d4an01370k
- Primary Topic
- Advanced biosensing and bioanalysis techniques
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Fundamental Research Funds for the Central Universities