A four-channel microfluidic model of the blood–brain and blood–cerebrospinal fluid barriers: fluid dynamics analysis

Abstract Brain-on-a-chip is an emerging field involving microfluidic devices capable of mimicking the structure and function of the human brain. Existing research often focuses on single barriers, such as the blood–brain barrier or blood–cerebrospinal fluid barrier (BCSFB). However, the brain has both barriers working together, and mimicking this dual system is crucial for better understanding of brain (patho)physiology. In this work, we present a four-channel microfluidic chip model that incorporates both the BBB and BCSFB, to reproduce physiologically correct architecture. Using computer simulations, we demonstrate that this model can mimic both healthy and diseased states by adjusting the shear stress experienced by the barriers, which is a key factor in their function. These findings offer valuable insights for designing future brain-on-a-chip devices with improved accuracy. This improved technology could contribute to wider advancements in tissue engineering and the study of brain function and diseases.