Protocol for in situ and in vivo dissection of mouse lung-draining lymph nodes
Lung-draining lymph nodes (LNs) play crucial roles in infection, inflammation, and cancer, but in situ and in vivo studying models are technically challenging. Here, we present a protocol for the in situ and in vivo dissection of mouse lung-draining LNs, ensuring postoperative survival. We describe steps for lymphadenectomy including localizing lung-draining LNs, open thoracotomy, and LN dissection. We then detail procedures for surgical sample preservation and postoperative care. This protocol offers broad applications in immunological and oncological research.
Authors
- Liqin Xiong (ORCID: https://orcid.org/0000-0001-8611-9691)
- Feng Yao (ORCID: https://orcid.org/0000-0002-9635-9581)
- Enshuo Zhang
- Yupeng Liu (ORCID: https://orcid.org/0000-0002-8652-1987)
- Gang Liu (ORCID: https://orcid.org/0000-0002-5180-3765)
- Haitang Yang (ORCID: https://orcid.org/0000-0002-8732-5910)
- Shuting Lu
- Xufeng Pan
- Bangchuan Zhao (ORCID: https://orcid.org/0000-0003-2892-3168)
Institutions
- Shanghai Jiao Tong University (CN)
- Shanghai Chest Hospital (CN)
- Nantong Tumor Hospital (CN)
Publication Details
- Journal
- STAR Protocols
- Published
- 2024-12-01
- DOI
- https://doi.org/10.1016/j.xpro.2024.103487
- Primary Topic
- Cancer Cells and Metastasis
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Natural Science Foundation of China
- Shanghai Chest Hospital