Sensitive Detection of 17β-Estradiol in Aqueous Medium via a Ternary Emission Molecularly Imprinted Sensor: A Comprehensive Scientific Research Experiment for Undergraduates

This study designed a comprehensive short laboratory course for senior undergraduate students to detect a type of estradiol (17β-estradiol, E2) in an aqueous medium using a ternary emission molecularly imprinted sensor. E2 is the most potent form of estrogen, and monitoring its residue levels in food and the environment is crucial for ensuring safety. Senior undergraduate students majoring in chemistry or related areas often need more practical laboratory experience and a deeper understanding of scientific research methodologies. To address this issue, this laboratory course employs molecular imprinting technology and quantum dot technology to develop ternary molecularly imprinted polymer (MIP) fluorescence sensors for the real-time detection of trace E2. The course includes literature review, sample pretreatment, polymer synthesis, material preparation, and material characterization, as well as data processing and analysis. These courses provide undergraduate students with complete research experience and facilitate the transition to postgraduate-level research work.