Seasonal mRNA Expression of Circadian Clock Genes in the Lizard Brain

Seasonally breeding animals undergo physiological and behavioral changes to time reproduction to occur during specific seasons. These changes are regulated by changing environmental conditions, which may be communicated to the brain using the central circadian clock. This clock consists of a daily oscillation in the expression of several core genes, including period (per), cryptochrome (cry), circadian locomotor output cycles kaput (clock), and basic helix-loop-helix ARNT-like protein 1 (bmal1). We began to examine seasonal regulation of four core circadian clock genes in a dissection of the reptile brain containing the hypothalamus-per1, cry1, bmal1 and clock. Our study focused on examining mRNA expression in the morning and compared levels between breeding and nonbreeding animals. We found that per1 and bmal1 mRNA expression was highest in the nonbreeding compared to breeding season in the anole hypothalamus. We also found that cry1 mRNA expression was higher in the female compared to the male anole hypothalamus. We found support for the idea that core circadian genes play a role in regulating changes between the seasons and/or sexes, although more work is needed to elucidate what processes might be differentially regulated. To our knowledge, this is the first examination of the expression of these four genes in the reptilian brain.