Effect of Heat Treatment on Gelatin Properties and the Construction of High Internal Phase Emulsions for 3D Printing

The effect of tilapia skin gelatin properties on the characteristics of high internal phase emulsions (HIPEs) and the quality of 3D printing remains unidentified. In this work, HIPEs were constructed by gelatin with various properties that were obtained by heat treatment. The results indicated that the gelatin undergoes degradation gradually with an increase in heating intensity. The highest values of intrinsic fluorescence intensity, surface hydrophobicity, and emulsification were obtained when the heating time was 5 h. The gel strength and hardness of gelatin hydrogels were negatively correlated with heat treatment temperature. HIPEs constructed by gelatin extracted at 70 °C demonstrated a suitable material for 3D printing. The storage modulus (G') and viscosity of HIPEs exhibited a similar tendency as the gel strength of gelatin. The microstructure of HIPEs revealed that gelatin established a gel network around oil droplets, and the higher G' of HIPEs corresponded to a more compact network structure. This study elucidated the correlation between the structure and properties of gelatin, offering essential insights for the formulation of HIPEs by natural gelatin, which is suitable for applications across several domains.