A novel transcription factor <scp>CsSNACA2</scp> plays a pivotal role within nitrogen assimilation in tea plants

Tea (Camellia sinensis) is a globally renowned economic crop, with organs such as leaves and buds utilized for consumption. As a perennial foliage crop, tea plants have high-nitrogen consumption and demand but exhibit relatively low nitrogen use efficiency. Exploring the genetic factors involved in nitrogen assimilation in tea plants could lead to improvements in both tea yield and quality. Here, we first conducted transcriptome sequencing on two tissues (roots and young leaves) under two different nitrate levels (0.2 and 2.5 mm KNO₃) and at six time points (0, 15, and 45 min; 2 and 6 h and 2 days). Differential gene expression patterns were observed for several genes that exhibited altered expression at 2 h. Clustering and enrichment analyses, along with co-expression network construction, provided evidence for the crucial involvement of CsSNACA2 in nitrogen assimilation. CsSNACA2 overexpression elicited pronounced phenotypic changes in nitrogen-deficient plants. Furthermore, CsSNACA2 suppressed the expression of CsNR (encoding nitrate reductase) and CsCLCa (encoding a <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"> <mml:mrow><mml:msubsup><mml:mi>NO</mml:mi> <mml:mn>3</mml:mn> <mml:mo>-</mml:mo></mml:msubsup> </mml:mrow> </mml:math> /H⁺ exchanger). Moreover, CsSNACA2 served as a downstream target of CsSPL6.1. In addition, we characterized Csi-miR156e and Csi-miR156k, which directly cleave CsSPL6.1. This study identified a transcription factor module participating in nitrogen assimilation in tea plants, providing a genetic foundation for future innovations in tea cultivar improvement. These results broaden our understanding of the genetic mechanisms governing nitrogen assimilation in dicotyledonous plants.