FEATURES OF REPARATIVE REGENERATION OF BONE TISSUE IN THE BAK-1000 IMPLANTATION ZONE IN COMBINATION WITH ANGIOSTIMULATED MSCS

An urgent problem in modern maxillofacial surgery remains the question of choosing the optimal osteoplastic material when eliminating diastasis of bone tissue, especially when replacing large defects. An active search and testing of new biocomposites that stimulate osteohistogenesis continues, assessing their effectiveness and safety. Aim of the study: characteristics of osteoregeneration after implantation of “BAK-1000” in combination with MSCs stimulated in the angiogenic direction in an experiment. Materials and methods. Experimental animals (Sprague Dawley rats, age 13–15 weeks, n = 30, ♂) in this study were randomly divided into two groups – control and experimental (15 animals in each). The first stage of the experiment is the cultivation of mesenchymal stem cells, the second is the creation and filling of bone defects using implantation material and autologous MSCs. Results. A histochemical study two months after implantation of the biocomposite in combination with MSCs revealed moderate development of signs of osteohistogenesis, pronounced neoangiogenesis and the formation of bright yellow crystals. Administration of BAC-1000 to animals in the control group demonstrated the formation of a connective tissue capsule around the implanted material with virtually no signs of osteohistogenesis and neoangiogenesis. Conclusions. In the experiment, the use of a biocomposite consisting of “BAK-1000” in combination with angiostimulated MSCs was tested. Based on a histochemical study, it was noted that it is ineffective in closing extensive bone tissue defects, however, additional cultivation of these cells on a matrix of osteoplastic materials can enhance the processes of osteohistogenesis and neoangiogenesis, inducing bone tissue metabolism and stimulating the formation of connective tissue in the diastasis zone, which may be the reason for further studies of such combinations.