Evaluation of Currently Available Laboratory Methods to Detect Terbinafine Resistant Dermatophytes Including a Gradient Strip for Terbinafine, <scp>EUCAST</scp> Microdilution E.Def 11.0, a Commercial Real‐Time <scp>PCR</scp> Assay, Squalene Epoxidase

The gradient strips, despite questionable essential agreement with EUCAST E.Def 11.0, can be an easy, fast and cheap method to screen terbinafine resistance among dermatophytes in clinical laboratories. The DermaGenius resistance PCR assay enables rapid detection of the most common substitutions in SQLE associated with terbinafine resistance. However, its inability to precisely determine specific substitutions on SQLE or identify new ones may pose a problem in the future. These limitations can be addressed by using SQLE sequencing or whole genome sequencing (WGS).