Protective effects of soybean peptides on H2O2-induced oxidative injury in IPEC-J2 cells

The purpose of the study was to demonstrate how soybean peptides (SBP) protect against H 2 O 2 -induced injury in intestinal porcine epithelial cells (IPEC-J2). SBP were prepared by protease hydrolysis, in which the molecular weights of 95.76% SBP were smaller than 3 kDa. Cell experiment included four groups: Control group (IPEC-J2 cells were treated with HGDMEM), SBP group (100 μg/mL SBP incubation for 13 h), H 2 O 2 treatment group (1 mM H 2 O 2 treatment for 1 h), SBP + H 2 O 2 group (100 μg/mL SBP pretreatment for 12 h followed by 1 mM H 2 O 2 treatment for 1 h). This study showed that that treatment with single 1 mM H 2 O 2 for 1 h significantly reduced cell viability to 52.99% ( p < 0.05), up-regulated Bax and Caspase-3 gene expressions ( p < 0.05), and down-regulated gene expressions of ZO-1, CAT, SOD1, HO-1 and Nrf2 ( p < 0.05), compared with the control group. However, pretreatment with SBP followed by H 2 O 2 inducement significantly increased cell viability to 72.99%, decreased cell apoptosis, increased SOD, CAT and GSH-Px activity ( p < 0.05), down-regulated Bax and Caspase-3 gene expressions ( p < 0.05), and up-regulated the gene expressions of ZO-1, Claudin-1, Occludin, catalase, glutathione GPX1, SOD1, HO-1, NQO1 and Nrf2, compared with the single H 2 O 2 –induced cells. According to the study, SBP pretreatment reduced H 2 O 2 -induced oxidative stress in cells and preserved the integrity of intestinal cells.