Identification of the bacterial phylotypes Bifidobacterium dentium, Actinomyces naeslundii, and Propionibacterium species in deep dental caries using fluorescence in situ hybridization

FISH is a simple, quick, and versatile approach for bacterial identification and quantification. It also has a high sensitivity, allowing it to detect single bacterial cells. This method can be applied directly to clinical samples and utilized as a rapid diagnostic tool for detecting the presence of newer phylotypes in deep dental caries.