Production of enzyme food using buckwheat leaves and sea tangle

The polyphenol content of common buckwheat, Tartary buckwheat, buckwheat leaves, sea tangle, sea mustard ears, and fusiformis were analyzed. Among these, buckwheat leaves exhibited the highest polyphenol content. Hot water extraction followed by treatment with plantase enzyme increased the polyphenol yield by 56% for buckwheat leaves and by 34% for sea tangle. Cytotoxicity test performed on 3T3-L1 pre-adipocytes for seaweeds and buckwheat leaves revealed no significant cytotoxic effects compared to that of the control group at 1 mg/mL. Additionally, when examining the effects of buckwheat leaf and sea tangle extracts on the pre-adipocytes differentiation, into adipocytes confirmed that buckwheat leaf extract inhibited fat differentiation at 10 mg/mL and sea tangle at 0.1 mg/mL. Buckwheat/sea tangle enzyme food had 6.5 times higher amylase activity, 27 times higher protease activity, and more than twice as high dietary fiber, polyphenol, and DPPH scavenging ability as brown rice enzyme foods. Buckwheat/sea tangle enzyme-supplemented food contained more than 30% more phlorotannins and 7.8% more alginic acid than brown rice enzyme-supplemented food.