Protocol for tyramide signal amplification immunohistochemical detection of Notch1 signaling in the vascular system
Notch signaling is a pivotal regulator in the vascular system that is essential for development, angiogenesis, and maintaining vascular homeostasis. Here, we present a protocol for tyramide signal amplification (TSA) immunohistochemistry, tailored explicitly for detecting Notch signaling components in vascular tissues. We describe steps for utilizing tailored antigen retrieval techniques, specific blocking solutions, and a complex of avidin/biotin-horseradish peroxidase conjugate with tyramide, along with optimized washing steps. For complete details on the use and execution of this protocol, please refer to Zhu et al.<sup>1</sup>.
Authors
- Dongyang Jiang (ORCID: https://orcid.org/0000-0001-6610-4249)
- Chong‐Rui Xu (ORCID: https://orcid.org/0000-0002-3474-2809)
- Guofu Zhu (ORCID: https://orcid.org/0000-0003-2823-0046)
- Ying Lin (ORCID: https://orcid.org/0000-0002-2270-272X)
- Leo Y. Luo (ORCID: https://orcid.org/0000-0002-3061-4287)
- Shekhar Singh (ORCID: https://orcid.org/0000-0001-9238-7940)
- Cailin Feng
- Wei‐Ming Li (ORCID: https://orcid.org/0000-0001-6155-0696)
- Wenliang Che
- Zeyu Wang (ORCID: https://orcid.org/0000-0002-0542-3534)
Institutions
- Tongji University (CN)
- Cardiovascular Institute of the South (US)
- Shanghai Tenth People's Hospital (CN)
- Stanford Medicine (US)
- Wuxi People's Hospital (CN)
- Stanford University (US)
Publication Details
- Journal
- STAR Protocols
- Published
- 2024-12-01
- DOI
- https://doi.org/10.1016/j.xpro.2024.103519
- Citations
- 2
- Primary Topic
- Developmental Biology and Gene Regulation
- Type
- article
- Field-Weighted Citation Impact
- 0.47
Funders
- Innovative Research Group Project of the National Natural Science Foundation of China
- National Natural Science Foundation of China