Polymorphisms in the type A <i>blaZ</i> gene as determinants of the cefazolin inoculum effect in <i>Staphylococcus aureus</i>
Plasmids containing the wild-type (WT) or mutant type A <i>blaZ</i> (<i>blaZ</i><sub>A</sub>) gene (mutations at codons 226, 229, or both 226 and 229) were constructed and transformed into <i>Staphylococcus aureus</i> RN4220, yielding the strains WT-blaZ, M226-blaZ, M229-blaZ, and MB-blaZ. The high-inoculum cefazolin MIC was significantly lower in MB-blaZ and M226-blaZ than in WT-blaZ but not in M229-blaZ, suggesting that the single nucleotide polymorphism at codon 226 in <i>blaZ</i><sub>A</sub> contributes to the cefazolin inoculum effect.
Authors
- Soon Ok Lee (ORCID: https://orcid.org/0000-0002-0399-2135)
- Jeong Nam Kim (ORCID: https://orcid.org/0000-0002-6825-0407)
- Shinwon Lee (ORCID: https://orcid.org/0000-0001-7652-7093)
- Jeong Eun Lee (ORCID: https://orcid.org/0000-0001-9900-3954)
- Sohee Park (ORCID: https://orcid.org/0000-0001-9243-6140)
- Sun Hee Lee (ORCID: https://orcid.org/0000-0003-2093-3628)
Institutions
- Pusan National University Hospital (KR)
- Pusan National University (KR)
Publication Details
- Journal
- Antimicrobial Agents and Chemotherapy
- Published
- 2024-12-10
- DOI
- https://doi.org/10.1128/aac.01106-24
- Primary Topic
- Antimicrobial Resistance in Staphylococcus
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Research Foundation
- Ministry of Science, ICT and Future Planning
- National Research Foundation of Korea