Riboflavin Spectrophotometric Determination with Zn(II) as a Choro Complex Anion using Cloud Point Extraction and HPLC in Different Drugs

Because riboflavin is becoming increasingly significant in the food, biological, and pharmaceutical fields, it is critical to accurately detect its content to guide nutritional health, diagnose and treat related diseases, conduct pharmacological research on riboflavin, and oversee the quality of food and drugs. A thorough application cloud point extraction and, liquid ion exchange in addition to HPLC techniques for the determination of riboflavin in pharmaceutical domains was provided. To produce an ion-pair association complex for RF with Zn(II) from HCl media and in the presence of Triton X-100 as non-ionic surfactant, the approach uses a liquid ion exchange process. The maximum wavelength was 295 nm. The influence of the ideal conditions on the separation process was examined: 95°C temperature, 15 min. of heating, 100µg/mL Zn(III) concentration, and TritonX-100 as the best surfactant. Within the concentration limit 5-1000μg/mL, the calibration curve is linear, with LOD of 6.07 μg/mL and LOQ of 18.38µg/mL. HPLC applied to compare the results with cloud point extraction and, liquid ion exchange in RF determination, they were identical.