Protocol for cellular RNA G-quadruplex profiling using G4RP.v2
The isolation of G-quadruplexes (G4s) from human cells using specific molecular tools constitutes an invaluable step forward in uncovering the biology of these higher-order DNA and RNA structures. Here, we present an improved version of the G4-RNA precipitation (G4RP) protocol developed to identify RNA G4s from human cancer cells. We describe steps for cell treatment and lysis, chemoprecipitation of G4s using TASQ tools, go/no-go steps, and quantitative reverse-transcription PCR (RT-qPCR) quantification and analysis. For complete details on the use and execution of this protocol, please refer to Mitteaux et al.<sup>1</sup>.
Authors
- Jérémie Mitteaux (ORCID: https://orcid.org/0000-0003-0839-553X)
- David Monchaud (ORCID: https://orcid.org/0000-0002-3056-9295)
Institutions
- Centre National de la Recherche Scientifique (FR)
- Université de Bourgogne (FR)
- Institut de Chimie (FR)
- Institut de Chimie Moléculaire de l'Université de Bourgogne (FR)
Publication Details
- Journal
- STAR Protocols
- Published
- 2024-12-01
- DOI
- https://doi.org/10.1016/j.xpro.2024.103480
- Citations
- 3
- Primary Topic
- DNA and Nucleic Acid Chemistry
- Type
- article
- Field-Weighted Citation Impact
- 0.70
Funders
- Communauté Université Grenoble Alpes
- European Commission
- Agence Nationale de la Recherche
- Centre National de la Recherche Scientifique