Amperometric Biosensor Based on Glutamate Oxidase to Determine Ast Activity

This work presents the development of an amperometric biosensor for detecting aspartate aminotransferase (AST) activity in biological fluids using a platinum disk electrode as the working transducer. Optimal concentrations of substrates (aspartate, α-ketoglutarate) and the coenzyme (pyridoxal phosphate) were determined to ensure efficient biosensor operation. A semi-permeable poly-m-phenylenediamine membrane was applied to enhance selectivity against electroactive interferents. The biosensor demonstrated good stability (storage, continuous operation, and production reproducibility) and analytical performance (sensitivity 8.56 nA/min for 50 U/L AST, LOD 1 U/L, linear range 1-110 U/L). Testing with real samples showed a high correlation (R = 0.989) with spectrophotometric analysis, supporting its potential for further applications.

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Publication Details

Journal
Sensors
Published
2024-12-10
DOI
https://doi.org/10.3390/s24247891
Citations
2
Primary Topic
Biosensors and Analytical Detection
Type
article
Field-Weighted Citation Impact
0.37

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article

Amperometric Biosensor Based on Glutamate Oxidase to Determine Ast Activity

О. О. Солдаткін, А. С. Сверстюк, Daryna Mruga, Vasyl Martsenyuk et al.
2 citations
Sensors
Biosensors and Analytical Detection
0.37
article

Amperometric Biosensor Based on Glutamate Oxidase to Determine Ast Activity

О. О. Солдаткін, А. С. Сверстюк, Daryna Mruga, Vasyl Martsenyuk, Aleksandra Kłos-Witkowska, Kseniia Berketa, S. V. Dzyadevych, Yurii Palianytsia
article en
2 citations

Abstract

This work presents the development of an amperometric biosensor for detecting aspartate aminotransferase (AST) activity in biological fluids using a platinum disk electrode as the working transducer. Optimal concentrations of substrates (aspartate, α-ketoglutarate) and the coenzyme (pyridoxal phosphate) were determined to ensure efficient biosensor operation. A semi-permeable poly-m-phenylenediamine membrane was applied to enhance selectivity against electroactive interferents. The biosensor demonstrated good stability (storage, continuous operation, and production reproducibility) and analytical performance (sensitivity 8.56 nA/min for 50 U/L AST, LOD 1 U/L, linear range 1-110 U/L). Testing with real samples showed a high correlation (R = 0.989) with spectrophotometric analysis, supporting its potential for further applications.

SensorsVol. 24(24)
National Academy of Sciences of Ukraine (UA), Taras Shevchenko National University of Kyiv (UA), National Technical University of Ukraine “Igor Sikorsky Kyiv Polytechnic Institute” (UA), Ternopil Ivan Pului National Technical University (UA), I.Horbachevsky Ternopil National Medical University (UA), University of Bielsko-Biała (PL), Institute of Molecular Biology and Genetics (UA)
Simons Foundation, National Research Foundation of Ukraine
Affordable and clean energy
Openalex Percentile: Top 42%
Biosensors and Analytical Detection
0.37
DOIOpen Access PDFOpenAlex
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Amperometric Biosensor Based on Glutamate Oxidase to Determine Ast Activity — О. О. Солдаткін, А. С. Сверстюк, et al. · Sensors (2024) | TGRS Research Map | TGRS