Comparative analysis of cell morphology in patient-paired primary human osteoblasts from the jaw and the fibula

Previous studies hint at possible differences in osteogenic, osteoimmunologic, and angiogenetic potential among primary human osteoblasts (HOBs) from different origins (iliac and alveolar bone) within the same patient. In this study, HOBs from the jaw and the fibula were investigated for the first time to gain further knowledge about the similarities and differences on the cellular morphological level. Patient-paired HOB cultures from the jaw and fibula of 14 patients (60.3 ± 11.1 years; male: 9; female: 5) were isolated and further processed. Cells were stained with Calcein and Hoechst 33342, and single-cell morphometric shape analysis was performed. For each osteoblast, the shape descriptors area, length, width, aspect ratio, circularity, roundness, and solidity were determined. A site-specific and a gender-specific comparison were conducted. None of the shape descriptors showed any significant differences between HOBs derived from the jaw and the fibula. The same applied to the gender-specific comparison between osteoblasts from female and male patients. Significant correlations between shape descriptors were found. HOBs from both bones possess a comparable cell shape, which might positively influence the ossification between the recipient and the donor bone. Since cell morphology often reflects cell function, both bones might exhibit comparable osteoblast behavior, adding to the favorable outcomes observed with free fibula flaps in reconstructive surgery.