Responses of hybrid aspen in vitro cultures to different proportions of red and blue light

Light is one of the most significant environmental factors affecting the growth of plants, as it is the driving force of photosynthesis. Among others, the red and blue light are the most relevant, as these spectral regions are absorbed by chlorophyll the most. In addition, red and blue light trigger specific photomorphogenic responses that allow plants to capture the available light efficiently. Accordingly, the proportion of red and blue light (R:B ratio) is considered one of the most important characteristics of light for plants, as optimal R:B provides balanced growth and photosynthesis. The aim of this study was to evaluate how the in vitro cultures of hybrid aspen (Populus tremula L. × P. tremuloides Michx.) are affected by different R:B ratios under fixed illumination intensity. We examined the growth characteristics of plantlets under wide spectrum LED luminaires with three different R:B proportions – 1:1, 7:3 and 9:1. While photosynthesis-related variables were significantly affected by light, the effect on morphology was less pronounced. Overall, increased proportions of red light decreased the photosynthetic performance of plantlets without giving significant benefits in the form of longer shoots that could be used to facilitate propagation effectiveness. Nonetheless, the effect of light treatment remains at least partially clones-specific and should be considered in case of further application for propagation purposes.