YES-DC-CP medium facilitates the isolation of <i>Aspergillus</i> section <i>Flavi</i> from acidic field soils

Aspergillus flavus and parasiticus agar (AFPA) medium is the most prevalent medium for the isolation of fungal strains of Aspergillus section Flavi. AFPA medium has been mainly used for the selection of the aflatoxin (AF) – producing strains of Aspergillus flavus and A. parasiticus. Recently we have developed an improved agar medium, named YES-DC-CP, for the isolation of Aspergillus section Flavi, regardless of the production of AF. In this study we adopted YES-DC-CP medium for the isolation of Aspergillus section Flavi strains from acidic tea field soils and compared the isolation efficacy with AFPA. As a result, YES-DC-CP medium was more suitable for the isolation. The isolated strains were chemically and genetically analyzed for the species identification. A. caelatus, one of the minor species of this section, was found in rather high frequencies.

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Publication Details

Journal
JSM Mycotoxins
Published
2024-12-10
DOI
https://doi.org/10.2520/myco.75-1-3
Primary Topic
Biofuel production and bioconversion
Type
article
Field-Weighted Citation Impact
0.00

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article

YES-DC-CP medium facilitates the isolation of Aspergillus section Flavi from acidic field soils

Kimiko Yabe, Tomohiro Furukawa, Yuhei Hirono, Masayo Kushiro et al.
JSM Mycotoxins
Biofuel production and bioconversion
article

YES-DC-CP medium facilitates the isolation of Aspergillus section Flavi from acidic field soils

Kimiko Yabe, Tomohiro Furukawa, Yuhei Hirono, Masayo Kushiro, Akiko Ogino, Y Tsukada, Yuhei Kanazashi, Yohei Yamagami
article en

Abstract

Aspergillus flavus and parasiticus agar (AFPA) medium is the most prevalent medium for the isolation of fungal strains of Aspergillus section Flavi. AFPA medium has been mainly used for the selection of the aflatoxin (AF) – producing strains of Aspergillus flavus and A. parasiticus. Recently we have developed an improved agar medium, named YES-DC-CP, for the isolation of Aspergillus section Flavi, regardless of the production of AF. In this study we adopted YES-DC-CP medium for the isolation of Aspergillus section Flavi strains from acidic tea field soils and compared the isolation efficacy with AFPA. As a result, YES-DC-CP medium was more suitable for the isolation. The isolated strains were chemically and genetically analyzed for the species identification. A. caelatus, one of the minor species of this section, was found in rather high frequencies.

JSM MycotoxinsVol. 75(1)
Fukui University of Technology (JP), National Agriculture and Food Research Organization (JP), Food Safety and Inspection Service (US), Institute of Fruit Tree and Tea Science (JP), Tea Research Institute (CN), Institute of Crop Science (JP)
Ministry of Agriculture, Forestry and Fisheries
Openalex Percentile: Top 79%
Biofuel production and bioconversion
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YES-DC-CP medium facilitates the isolation of <i>Aspergillus</i> section <i>Flavi</i> from acidic field soils — Kimiko Yabe, Tomohiro Furukawa, et al. · JSM Mycotoxins (2024) | TGRS Research Map | TGRS