FACS-based detection of extracellular ASC specks from NLRP3 inflammasomes in inflammatory diseases
We validated fluorescence-activated cell sorting as a reliable method for detecting ASC/NLRP3 specks in human sera, with potential diagnostic and monitoring applications in certain systemic autoinflammatory diseases.
Authors
- Sinisa Savic (ORCID: https://orcid.org/0000-0001-7910-0554)
- Leon Chang (ORCID: https://orcid.org/0009-0003-5223-7616)
- Heledd Jarosz-Griffiths (ORCID: https://orcid.org/0000-0001-5154-4815)
- D. Peckham (ORCID: https://orcid.org/0000-0001-7723-1868)
- Samuel Lara‐Reyna (ORCID: https://orcid.org/0000-0002-9986-5279)
- Michael McDermott (ORCID: https://orcid.org/0000-0002-1015-0745)
- James A. Poulter (ORCID: https://orcid.org/0000-0003-2048-5693)
- Alice Ibbotson
- Joanne Topping
Institutions
- University of Leeds (GB)
- Chapel Allerton Hospital (GB)
- St James's University Hospital (GB)
- NIHR Leeds Musculoskeletal Biomedical Research Unit (GB)
Publication Details
- Journal
- Clinical & Experimental Immunology
- Published
- 2024-12-06
- DOI
- https://doi.org/10.1093/cei/uxae117
- Citations
- 5
- Primary Topic
- Inflammasome and immune disorders
- Type
- article
- Field-Weighted Citation Impact
- 1.17
Funders
- Cancer Research UK
- National Institute for Health and Care Research
- Department of Health and Social Care
- Leeds Biomedical Research Centre