Quantification of lysosomal labile Zn2+ and monitoring of Zn2+ efflux using a small-molecule–protein hybrid fluorescent probe
Lysosomal labile Zn<sup>2+</sup> levels have been unclear. By targeting a small-molecule fluorescent Zn<sup>2+</sup> probe, ZnDA-3H, to lysosomes via VAMP7-Halo, the lysosomal labile Zn<sup>2+</sup> concentration was determined to be 1.9 nM in HeLa cells. Furthermore, ZnDA-3H enabled direct visualization of the Zn<sup>2+</sup> efflux from the lysosomes to cytosol upon TRPMLs activation.
Authors
- Yuyin Du (ORCID: https://orcid.org/0000-0003-0795-3405)
- Rong Liu (ORCID: https://orcid.org/0000-0001-8360-2784)
- A. Yu. Soloviev
- Ellen Sung
- Shin Mizukami (ORCID: https://orcid.org/0000-0002-2292-8606)
- Toshiyuki Kowada (ORCID: https://orcid.org/0000-0002-6623-6505)
- Toshitaka Matsui
Institutions
- Tohoku University (JP)
Publication Details
- Journal
- Journal of Inorganic Biochemistry
- Published
- 2024-12-10
- DOI
- https://doi.org/10.1016/j.jinorgbio.2024.112811
- Primary Topic
- Trace Elements in Health
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Konica Minolta Imaging Science Foundation
- Takeda Science Foundation
- Japan Agency for Medical Research and Development
- Ministry of Education, Culture, Sports, Science and Technology
- Nakatani Foundation for Advancement of Measuring Technologies in Biomedical Engineering
- Japan Society for the Promotion of Science
- Core Research for Evolutional Science and Technology