Isolation and Culturing of Primary Neurons from Newborn Mouse Cortex Tissue

Objective: This study aimed to establish a reliable protocol for obtaining healthy and long-lived cortical neurons from newborn mice, providing a valuable model for studying neuronal function. Materials and Methods: Cortical regions of P0 mice were isolated and healthy neurons were obtained by enzymatic and mechanical dissociation. On the seventh day of incubation, the presence of neurons was detected by staining with neuron-specific antibodies. Transgenic mice expressing fluorescent proteins specific to neurons, glia and oligodendrocytes were also used for culture. Results: Almost all the neurons had adhered to the petri dish bottom by the second hour of incubation. Most of the neurons were healthy and started to grow extension quickly. Conclusion: Neuron cultures are an important tool in research and are invaluable for studying the behaviour of cells. Nanoparticles facilitate genetic manipulation of these cultures for various biotechnological applications. In particular, they have great potential in areas such as the delivery of genetic material into cells, drug delivery and targeted treatment methods. Such techniques have the potential to open up new avenues for the study and treatment of neurological diseases.