Large-scale production of Mansonella perstans infective larvae from engorged Culicoides milnei
We revealed suitable conditions for the collection and maintenance of engorged <i>Culicoides</i> midges allowing the large-scale production of <i>M. perstans</i> L3. This procedure will provide a platform to produce sufficient parasite material that will facilitate <i>in vitro</i> cultures and the establishment of a murine model of <i>M. perstans</i>, which is important for in-depth investigation of the filarial biology and screening of novel drugs that are effective against this ivermectin-resistant nematode.
Authors
- Fanny Fri Fombad (ORCID: https://orcid.org/0000-0003-2575-9884)
- Rene Ebai
- Chi Anizette Kien (ORCID: https://orcid.org/0000-0001-9632-2028)
- Juluis Visnel Foyet (ORCID: https://orcid.org/0000-0001-6564-9167)
- Manuel Ritter (ORCID: https://orcid.org/0000-0001-8667-2067)
- Abdel Jélil Njouendou (ORCID: https://orcid.org/0000-0002-6336-6160)
- Peter Enyong (ORCID: https://orcid.org/0000-0001-8399-6031)
- Lucy Cho Nchang (ORCID: https://orcid.org/0009-0008-0546-0109)
- Franck Noel Nietcho
- Chefor Magha
- Narcisse Victor Tchamatchoua Gandjui (ORCID: https://orcid.org/0000-0003-3314-4863)
- Relindis Ekanya (ORCID: https://orcid.org/0000-0002-8358-1810)
- Achim Hoerauf (ORCID: https://orcid.org/0000-0002-8671-3831)
- Samuel Wanji (ORCID: https://orcid.org/0000-0003-0022-8366)
- Frederick Esofi (ORCID: https://orcid.org/0009-0005-4764-8431)
- Emmanuel Ouam
- Anna Ning Ntuh (ORCID: https://orcid.org/0009-0006-9681-0092)
- Valerine Chawa Chunda (ORCID: https://orcid.org/0009-0003-1265-7835)
Institutions
- University of Buea (CM)
- University Hospital Bonn (DE)
- German Center for Infection Research (DE)
Publication Details
- Journal
- Frontiers in Tropical Diseases
- Published
- 2024-12-10
- DOI
- https://doi.org/10.3389/fitd.2024.1391823
- Citations
- 1
- Primary Topic
- Parasitic Diseases Research and Treatment
- Type
- article
- Field-Weighted Citation Impact
- 0.39