TỐI ƯU HÓA ĐIỀU KIỆN CHIẾT XUẤT BÀI THUỐC SINH MẠCH TÁN BẰNG PHƯƠNG PHÁP BỀ MẶT ĐÁP ỨNG

The aim of the study is to optimize the extraction process from Shengmai San using the Response Surface Methodology (RSM). The factors of ethanol concentration, temperature, and extraction time have an impact on the performance of extraction (PE) and the anti-free radical activity DPPH %I (p<0.05). The method used was regression analysis of experimental data, resulting in a second-degree polynomial model with a relatively strong correlation to variance analysis that is statistically significant (p<0.05), and high R2R^2R2, Radj2R^2_{adj}Radj2​ values, indicating a reliable and suitable model for real-world application. The results determined the optimal conditions of RSM as follows: ethanol concentration of 67.0%; temperature of 62.2°C; and extraction time of 2.9 hours. The expected optimal values for PE and DPPH %I under these conditions are 32.6% and 78.4%, respectively. To align with practical application, the optimal conditions for extraction from Shengmai San are an ethanol concentration of 70%, a temperature of 60°C, and an extraction time of 3 hours. The PE and DPPH %I values obtained were 32.4% and 78.1%, respectively, with no significant deviation from the RSM model results (p>0.05).